: Guidance on using the XFluor4 or Magellan data reduction packages for assay analysis.
The is not just for emergencies. Use it to build a Preventative Maintenance (PM) schedule:
Understanding the internal hardware of the Tecan Safire 2 is critical for optimizing assay parameters and ensuring optimal signal-to-noise ratios. Monochromator Technology tecan safire 2 manual pdf
Launch the Magellan software interface. The software will perform a Power-On Self-Test (POST) to verify monochromator calibration and mechanical initialization.
Tecan Group Ltd. has migrated most legacy documentation to their . You will need a free account (registered with your institutional email). : Guidance on using the XFluor4 or Magellan
The Tecan Safire 2 is a highly versatile, monochromator-based microplate reader engineered for complex life science research and high-throughput screening. Known for its modular design, this instrument allows laboratories to perform absorbance, fluorescence, and luminescence assays without the need for fixed optical filters.
: Allows for the addition of specialized modules like fluorescence polarization and multi-channel luminescence. has migrated most legacy documentation to their
The Safire 2 supports complex protocols like kinetic scans, well scanning (multiple reads per well), and spectral scanning. The manual explains how to configure the i-control software (version 1.x or 2.x) to set integration times, settle times, and number of flashes—parameters that directly impact signal-to-noise ratio.
, which hosts documentation for their liquid handling and microplate reader series. : For legacy instruments like the
Set the PMT (photomultiplier tube) gain. Use the "Optimal" or "Auto-Gain" feature to prevent signal saturation in highly concentrated samples while maintaining sensitivity in low-signal wells.
The Tecan Safire 2 is primarily driven by Tecan’s proprietary Magellan software package. Follow these steps to execute a basic microplate scan. 1. System Initialization